Anticancer activity of terpenoid saponin extract of Psidium guajava on MCF-7 cancer cell line using DAPI and MTT assays

Psidium guajava (Guava) could be an ancient remedy used for a variety of health conditions. Research suggests that guava fruits and leaves might have advantages. Guava is the tropical tree with yellowish-green skin fruits, widely grown in Central America Asia. Individuals use leaf tea as treatment gastric symptoms many countries, together India China. In different like Mexico, individuals historically flesh fruit to heal wounds. extract had shown anticancer, antidiabetic, antispasmodic anthelmintic effects various research studies. our present study, terpenoid saponin, novel molecule isolated from fraction studied anticancer activity using 4′, 6-diamidino-2-phenylindole (DAPI) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays against MCF-7 breast cancer cell line.  Terpenoid saponin >60% apoptotic DAPI staining assay normal line >99% average %inhibition at 400 μg/ml which significant result. Thus, we suggest further studies potential usage. Key words: leaves, guajava, glycoside, (DAPI), (MTT), assay.